《植物生理学报》 2018, 54(6): 1018-1028

玉米PLCs基因家族鉴定及表达谱分析

董洁静^1,2,3,4, 徐晶宇^1,2,4, 林俊俊^1,2,3,4, 贺琳^1,2,4, 赵长江^1,2,3,4,*, 李佐同^1,2,3,4,*

¹黑龙江八一农垦大学农学院, 黑龙江大庆163319; ²黑龙江省现代农业栽培技术与作物种质改良重点实验室, 黑龙江大庆163319; ³黑龙江省秸秆资源化利用工程技术研究中心, 黑龙江大庆163319; ⁴黑龙江省普通高校寒地作物种质改良与栽培重点实验室, 黑龙江大庆163319

通信作者：赵长江；E-mail: zhaocj15@126.com, lxg6401999@163.com

摘　要：

磷脂酶C (phospholipase C, PLC)是一类能够水解磷脂的酶, 作为磷脂酶家族中的一员, 在调节植物生长及响应逆境胁迫中发挥重要作用。本文通过对ZmPLCs蛋白的系统发育、其基因结构及启动子中的逆境相关顺式作用元件数量, 以及基因的组织特异性表达模式等生物信息学进行研究; 并用NaHCO₃和低温处理玉米转录组数据, 构建ZmPLCs基因表达谱。结果显示, 在玉米基因组水平上共鉴定出9个玉米PLCs基因, 基于玉米PLCs水解底物的不同, 9个ZmPLCs基因被分为2个亚族, 分别定位在玉米的1、2、3、5和9号染色体上。Zm-PI-PLCs编码蛋白包含典型的X、Y和C₂结构域, ZmNPCs蛋白包含典型的磷酸酯酶结构域, ZmPLCs蛋白N端由延长链和螺旋结构组成, 多定位于线粒体和叶绿体。ZmPLCs基因替换发现ZmPLCs家族可能是通过变异进行扩增的。在9个ZmPLCs基因启动子上包括脱落酸、盐和低温等激素和非生物胁迫相关元件高度富集, 转录组数据分析ZmPI-PLC2和ZmNPC2上调响应NaHCO₃, ZmPI-PLC5和ZmNPC3上调响应低温, ZmPI-PLC3和Zm-PI-PLC4与玉米根系发育有关, 这与基因表达模式相印证, 进一步表明玉米PLCs基因参与植物对多种非生物逆境的响应, 以及作物对非生物抗性利用的潜在应用价值。

关键词：玉米; PLC; 非生物逆境; 表达分析; 生物信息学

收稿：2018-02-07   修定：2018-05-29

资助：黑龙江省自然科学基金(QC2016036)和黑龙江农垦总局科技攻关项目(HNK135-02-05)。

Genome-wide identification and expression analysis of PLCs gene family in maize (Zea mays)

DONG Jie-Jing^1,2,3,4, XU Jing-Yu^1,2,4, LIN Jun-Jun^1,2,3,4, HE Lin^1,2,4, ZHAO Chang-Jiang^1,2,3,4,*, LI Zuo-Tong^1,2,3,4,*

¹College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China; ²Heilongjiang Provincial Key Laboratory of Modern Agricultural Cultivation and Crop Germplasm Improvement, Daqing, Heilongjiang 163319, China; ³Heilongjiang Engineering Technology Research Center for Crop Straw Utilization, Daqing, Heilongjiang 163319, China; ⁴Key Laboratory of Crop Germplasm Improvement and Cultivation in Cold Regions of Heilogjiang Province Education Department, Daqing, Heilongjiang 163319, China

Corresponding author: ZHAO Chang-Jiang; E-mail: zhaocj15@126.com, lxg6401999@163.com

Abstract:

Phospholipase is a class of enzymes hydrolyzing phospholipids. Phospholipase C (PLC), as a member of the phospholipase family, plays an important role in regulating plant growth and response to stress. To clear the function of ZmPLCs, the series analysis were performed by biological information methods, including phylogenetic and conserved motifs of ZmPLCs, gene structurer and tissue-specific expression patterns of Zm-PLCs, and number of cis-acting elements involved in hormones and stresses in gene promoters. And the stress profile of ZmPLCs was constructed through the data of transcriptomes of maize tissues under NaHCO₃ and low temperature treatments respectively. In this study, based on the differences in hydrolysis substrates of maize PLCs enzymes, all ZmPLCs genes were divided into 2 subfamilies, located on chromosome 1, 2, 3, 5 and 9, respectively. The ZmPI-PLCs proteins had typical X, Y and C₂ motif, ZmNPCs encoded proteins hand typical Phosphoesterase motif. ZmPI-PLCs complicated N-terminal made up of extending chains and helixes, they localized in mitochondria and chloroplast. Gene replacement studies showed that the member amplification of maize PLCs family were driven by mutation. In the ZmPLCs family, there were a large number of the cis-element related to hormones and abiotic stress, for example abscisic acid (ABA), salt (NaCl) and low temperature were highly enriched in gene promoters, supported by the expression profile of maize transcriptome on saline and alkaline and low temperature treatment. ZmPI-PLC2 and ZmNPC2 were up-regulated response to NaHCO₃, ZmPI-PLC5 and ZmNPC3 were up-regulated response to low temperatures, ZmPI-PLC3 and ZmPI-PLC4 were related to maize root development. All above results suggested that these maize PLCs genes may respond to a variety of abiotic stresses and possessed potential functions in improving abiotic stress resistance.

Key words: maize (Zea mays); PLC; abiotic stress; expression profile; bioinformatic analysis